Journal: International Journal for Parasitology: Drugs and Drug Resistance

Article Title: In vitro activity, safety and in vivo efficacy of the novel bumped kinase inhibitor BKI-1748 in non-pregnant and pregnant mice experimentally infected with Neospora caninum tachyzoites and Toxoplasma gondii oocysts

doi: 10.1016/j.ijpddr.2021.05.001

Figure Lengend Snippet: Structure and in vitro activities of BKI-1748 against N. caninum (Nc-β-gal) and T. gondii (Tg-β-gal). (A) molecular structure of BKI-1748, MW = molecular weight; EC 50 = concentration of half maximal proliferation inhibition; *toxicity levels against human foreskin fibroblasts (HFF) and zebrafish embryos were determined previously [22]. (B,C) Dose-response curves for Neospora and Toxoplasma tachyzoites grown in HFF.

Article Snippet: Human foreskin fibroblasts (HFF; PCS-201-010TM) and BALB/c dermal fibroblasts (CELLNTEC AG, Bern, Switzerland) were maintained as described ( ).

Techniques: In Vitro, Molecular Weight, Concentration Assay, Inhibition

Journal: International Journal for Parasitology: Drugs and Drug Resistance

Article Title: In vitro activity, safety and in vivo efficacy of the novel bumped kinase inhibitor BKI-1748 in non-pregnant and pregnant mice experimentally infected with Neospora caninum tachyzoites and Toxoplasma gondii oocysts

doi: 10.1016/j.ijpddr.2021.05.001

Figure Lengend Snippet: Litter size, parasite burden, neonatal and postnatal mortality rates of N. caninum infected mice treated with BKI-1748.

Article Snippet: Human foreskin fibroblasts (HFF; PCS-201-010TM) and BALB/c dermal fibroblasts (CELLNTEC AG, Bern, Switzerland) were maintained as described ( ).

Techniques: Infection

Journal: Acta Dermato-Venereologica

Article Title: Novel Detection and Clinical Utility of Serum-Derived Extracellular Vesicle in Angiosarcoma

doi: 10.2340/actadv.v105.40902

Figure Lengend Snippet: miR-3926, miR-5703, miR-3925-5p, and miR-184 were upregulated in angiosarcoma (AS) cell lines. Expression of microRNAs (miRNAs) in an AS cell line (ISO-HAS B) and benign human endothelial cell lines (HUVEC and HDMVECn.) were compared by quantitative reverse transcription PCR. ISO-HAS B showed significant upregulation of miR-5703 ( P adj = 0.0078 and P adj = 0.0261, respectively) and miR-184 ( P adj = 0.0214 and P adj = 0.0053, respectively) in comparison with HUVEC and HDMVECn (F value for each one-way ANOVA analysis was 12.14 and 14.06, respectively). ISO-HAS B also showed a tendency to upregulations in miR-3926 and miR-3925-5p, while the differences were not significant (F value for each one-way ANOVA analysis was 3.154 and 3.237, respectively). The experiment was performed 3 times with triplicate samples for each cell line. HUVEC: human umbilical vein endothelial cell; HDMVECn: human dermal microvascular endothelial cells neonatal. * p < 0.05, ** p < 0.01.

Article Snippet: Commercial human umbilical vein endothelial cells (HUVEC) (ATCC ® , Manassas, VA, USA, PCS-100-010TM) and human dermal microvascular endothelial cells neonatal (HDMVECn) (ATCC ® , PCS-110-010TM) were cultured in vascular cell basal medium (ATCC ® , PCS-110-030TM) supplemented with an Endothelial Cell Growth Kit-VEGF (ATCC ® , PCS-110-041TM).

Techniques: Expressing, Reverse Transcription, Comparison

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Evaluation of cell toxicity and DNA and protein binding of green synthesized silver nanoparticles.

doi: 10.1016/j.biopha.2018.02.069

Figure Lengend Snippet: Fig. 5. Cytotoxicity of AgNPs in normal human primary fibroblasts. Cell viability de- termined by MTS assay of fibroblasts challenged with increasing concentrations of AgNPs for 24 h. Data normalized against the control treated with 1% tea extract and expressed as mean ± SEM of three independent assays. **** indicates statistically significant differ- ence for p < 0.01 (as compared to control).

Article Snippet: Primary Dermal Normal Human Fibroblasts (Neonatal) (American Type Culture Collection (ATCC) - PCS-201-010TM) were grown as previously described [34].

Techniques: MTS Assay, Control